The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.

نویسندگان

  • S D Nuttall
  • S E Deutschel
  • R A Irving
  • J A Serrano-Gomicia
  • M L Dyall-Smith
چکیده

We have designed a gene cassette for expression of the bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin. Recombinant ShBle was purified by a one-step affinity-chromatography procedure as a correctly folded, dimeric protein. ShBle thus provides a useful haloarchaeal selectable marker and represents the first non-halophilic and soluble heterologous protein to be expressed in the Haloarchaea.

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عنوان ژورنال:
  • The Biochemical journal

دوره 346 Pt 2  شماره 

صفحات  -

تاریخ انتشار 2000